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2014
Olagunju, A, Siccardi M, Amara A, Jevtovic D, Kusic J, Owen A, Dragovic G.  2014.  CYP2B6 516G>T (rs3745274) and smoking status are associated with efavirenz plasma concentration in a Serbian cohort of HIV patients. Therapeutic Drug Monitoring. 36(6):734-8. AbstractWebsite

Background: Genetic factors have been associated with efavirenz (EFV) plasma concentrations in different populations. In this study, we investigated the effects of CYP2B6 516G>T (rs3745274), CYP2B6 c.485-18C>T (rs4803419), CAR c.540C>T (rs2307424), CAR c.152-1089T>C (rs3003596) and smoking status in a cohort of Serbian HIV patients.

Methods: HIV-positive patients, all Cacausian, were recruited from the HIV/AIDS Center at the Infectious and Tropical Diseases Hospital, University of Belgrade Teaching Hospital, Belgrade, Serbia. Mid-dose (10-14 hours post dose) EFV plasma concentration was determined using a validated liquid chromatography/tandem mass spectrometry method. Genotyping for CYP2B6 516G>T (rs3745274), CYP2B6 c.485-18C>T (rs4803419), CAR c.540C>T (rs2307424) and CAR c.152-1089T>C (rs3003596) was conducted using allelic discrimination real-time PCR assay. One-way analysis of variance (ANOVA), Mann-Whitney test, Pearson or Spearman correlation and multiple linear regression were used for data analysis.

Results: Minor allele frequencies (MAF) were similar to frequencies reported in other European populations. The overall mean (95% CI) plasma EFV concentration was 2800 ng/ml (2460, 3140). Significant differences between patients based on CYP2B6 516G>T (rs3745274) genotypes were observed: GG (n = 60), 2320 ng/ml (2160, 2480); GT (n = 30), 3230 ng/ml (2790, 3670); and TT (n = 2), 10700 ng/ml (range: 6170, 15300) (p = 2.0 X 10-17). In multivariate linear regression analysis, CYP2B6 516G>T (rs3745274) ([beta] = 1770 ng/ml (1230, 2310), p < 0.0001) and smoking status ([beta] = -464 ng/ml (-1250, -43.3), p = 0.038) were independently associated with plasma EFV concentrations.

Conclusions: The effects of CYP2B6 516G>T (rs3745274) and smoking status on EFV plasma concentration in the Serbian population have been established for the first time.

Olagunju, A, Siccardi M, Amara A, Jevtovic D, Kusic J, Owen A, Dragovic G.  2014.  CYP2B6 516G>T (rs3745274) and smoking status are associated with efavirenz plasma concentration in a Serbian cohort of HIV patients. Therapeutic Drug Monitoring. 36(6):734-8. Abstract

BACKGROUND: Genetic factors have been associated with efavirenz (EFV) plasma concentrations in different populations. In this study, we investigated the effects of CYP2B6 516G>T (rs3745274), CYP2B6 c.485-18C>T (rs4803419), CAR c.540C>T (rs2307424), CAR c.152-1089T>C (rs3003596), and smoking status in a cohort of Serbian patients with HIV.

METHODS: Patients with HIV positive, all whites, were recruited from the HIV/AIDS Center at the Infectious and Tropical Diseases Hospital, University of Belgrade Teaching Hospital, Belgrade, Serbia. Mid dose (10-14 hours after dose) EFV plasma concentration was determined using a validated liquid chromatography/tandem mass spectrometry method. Genotyping for CYP2B6 516G>T (rs3745274), CYP2B6 c.485-18C>T (rs4803419), CAR c.540C>T (rs2307424), and CAR c.152-1089T>C (rs3003596) was conducted using allelic discrimination real-time polymerase chain reaction assay. One-way analysis of variance, Mann-Whitney test, Pearson or Spearman correlation, and multiple linear regression were used for data analysis.

RESULTS: Minor allele frequencies were similar to frequencies reported in other European populations. The overall mean (95% confidence interval) plasma EFV concentration was 2800 ng/mL (2460-3140). Significant differences between patients based on CYP2B6 516G>T (rs3745274) genotypes were observed: GG (n = 60), 2320 (range, 2160-2480) ng/mL; GT (n = 30), 3230 (range, 2790-3670) ng/mL; and TT (n = 2), 10,700 (range, 6170-15,300) ng/mL (P = 2.0 × 10). In multivariate linear regression analysis, CYP2B6 516G>T (rs3745274) [β = 1770 (1230 to 2310) ng/mL, P < 0.0001] and smoking status [β = -464 (-1250 to -43.3) ng/mL, P = 0.038] were independently associated with plasma EFV concentrations.

CONCLUSIONS: The effects of CYP2B6 516G>T (rs3745274) and smoking status on EFV plasma concentration in the Serbian population have been established for the first time.

Olagunju, A, Schipani A, Siccardi M, Egan D, Khoo S, Back D, Owen A.  2014.  CYP3A4*22 (c.522-191 C>T; rs35599367) is associated with lopinavir pharmacokinetics in HIV-positive adults. Pharmacogenetics and Genomics. 24(9):459-63. AbstractWebsite

The CYP3A4*22 (c.522-191 C>T; rs35599367) single nucleotide polymorphism has been associated with lower CYP3A4 mRNA expression and activity. We investigated the association of CYP3A4*22 with the pharmacokinetics of lopinavir through a population pharmacokinetic approach. The minor allele frequency for CYP3A4*22 was 0.035, and seven of 375 patients had a combination of CYP3A4*22 and SLCO1B1 521T>C alleles. Lack of information on the ethnicity in this cohort should be considered as a limitation. However, in the final model, the population clearance was 5.9 l/h and patients with CYP3A4*22/*22 had 53% (P=0.023) lower clearance compared with noncarriers. In addition, the combined effect of CYP3A4*22 with SLCO1B1 521T>C (previously shown to be associated with lopinavir plasma concentration) was analysed. We observed a 2.3-fold higher lopinavir trough concentration (Ctrough) in individuals with CYP3A4*22/*22, a 1.8-fold higher Ctrough with SLCO1B1 521CC and a 9.7-fold higher Ctrough in individuals homozygous for both single nucleotide polymorphisms, compared with noncarriers. A simulated dose-reduction scenario showed that 200/100 mg lopinavir/ritonavir was adequate to achieve therapeutic concentration in individuals with CYP3A4*22/*22 alone or in combination with SLCO1B1 521CC. These data further our understanding of the genetic basis for variability in the pharmacokinetics of lopinavir.

Olagunju, A, Schipani A, Siccardi M, Egan D, Khoo S, Back D, Owen A.  2014.  CYP3A4*22 (c.522-191 C>T; rs35599367) is associated with lopinavir pharmacokinetics in HIV-positive adults. Pharmacogenetics and Genomics. 24(9):459-463. Abstract

The CYP3A4*22 (c.522-191 C>T; rs35599367) single nucleotide polymorphism has been associated with lower CYP3A4 mRNA expression and activity. We investigated the association of CYP3A4*22 with the pharmacokinetics of lopinavir through a population pharmacokinetic approach. The minor allele frequency for CYP3A4*22 was 0.035, and seven of 375 patients had a combination of CYP3A4*22 and SLCO1B1 521T>C alleles. Lack of information on the ethnicity in this cohort should be considered as a limitation. However, in the final model, the population clearance was 5.9 l/h and patients with CYP3A4*22/*22 had 53% (P=0.023) lower clearance compared with noncarriers. In addition, the combined effect of CYP3A4*22 with SLCO1B1 521T>C (previously shown to be associated with lopinavir plasma concentration) was analysed. We observed a 2.3-fold higher lopinavir trough concentration (Ctrough) in individuals with CYP3A4*22/*22, a 1.8-fold higher Ctrough with SLCO1B1 521CC and a 9.7-fold higher Ctrough in individuals homozygous for both single nucleotide polymorphisms, compared with noncarriers. A simulated dose-reduction scenario showed that 200/100 mg lopinavir/ritonavir was adequate to achieve therapeutic concentration in individuals with CYP3A4*22/*22 alone or in combination with SLCO1B1 521CC. These data further our understanding of the genetic basis for variability in the pharmacokinetics of lopinavir.

Oluwadare, CO, Ojo O.  2014.  Database creation for tenement rate collection: The role of GIS. The Pacific Journal of Science and Technology. 15(1):190-199.
Adebayo P. Idowu, Olajide O. Adeosun, KWO.  2014.  Dependable Online Appointment Booking System for NHIS Outpatient in Nigerian Teaching Hospitals. International Journal of Computer Science & Information Technology. 6(4):59-73.
Folayan, MO, Khami MR, Folaranmi N, Orenuga O, Popoola BO, Olatosi O, Ligali TO, Sofola OO, Adeniyi AA, Esan A, Osaguona A.  2014.  Determinants of preventive dental practice for children among final year dental students in Nigeria . International Journal of Paediatric Dentistry . 24(1):43-50.
Abereijo, IO.  2014.  Developing Entrepreneurial Competences in University Lecturers: Obafemi Awolowo University Experience. Kenya College of Accountancy University Journal of Business Management (KJBM). 6(1):30-42.
  2014.  Development of a Multimedia Mobile Dictionary of Greeting Terms for Nigerian Indigenous Languages. Proceedings of 5th International Conference on Mobile e-Services. , Nigeriapapere5xvi.pdf
Asahiah, FO.  2014.  Development of a Standard Yorùbá Digital Text Automatic Diacritic Restoration System. (Dr. O A Odejobi, Prof. E A Adagunodo, Eds.)., Ile-Ife, Nigeria: Obafemi Awolowo UniversityAsahiah _PhD_Thesis
Satope, OO, Boboye IO, Akinwale OB, Kehinde LO, Asubiojo OI.  2014.  Development of a Suit of Virtual Experiments for Physics and Chemistry Undergraduate Laboratories. 121st ASEE Annual Conference & Expostion. :PaperID:#8876., Indianapolis: ASEEdevelopment-of-a-suite-of-virtual-experiments-for-physics-and-chemistry-undergraduate-laboratories.pdf
Haire, B, Folayan MO, Fleming J.  2014.  Development of guidelines for the conduct of HIV research monitoring by ethics committees in Nigeria. African Journal of Reproductive Health . 18(3):66-73.
Folayan, MO, Odetoyingbo M, Brandon B, Harrison A.  2014.  Differences in sexual behaviour and sexual practices of adolescents in Nigeria based on sex and self-reported HIV status. BMC Reproductive Health . 11:83.doi:10.1186/1742-4755-11-83.
Oladokun, TT, Aluko BT.  2014.  Dispute Resolution in Corporate Multi Tenanted Property Management: A case Study. Journal of Corporate Real Estate . 16(1):22-32.
Vitali, L. A., Petrelli, D., Lamikanra, A., Prenna, M., Akinkunmi EO.  2014.  Diversity of Antibiotic Resistance Genes and Staphylococcal Cassette Chromosome mec elements in Faecal Isolates of Coagulase-Negative Staphylococci from Nigeria. . BMC Microbiolgy. 14(106)
Adelokun, AS.  2014.  Dr. . Educational Management in Africa.Papers in Honour of Professor John Iheukwumere Nwankwo.. , Ibadan: University of Ibadan.
Adelokun, AS.  2014.  Dr. . Educational Management in Africa.Papers in Honour of Professor John Iheukwumere Nwankwo.. , Ibadan: University of Ibadan.
Yakubu, A, Folayan MO, Sani-Gwarzo N, Nguku P, Peterson K, Brandon B.  2014.  The Ebola outbreak in Western Africa-ethical obligations for care . Journal of Medical Ethics . :doi:10.1136/medethics-2014-102434..
Okoya, AA, Sonaike TO, Adekunle OK.  2014.  Effect of Application rates of selected natural pesticides on soil biochemical parameters and litter decomposition. African Journal of Agricultural Research. 9(50):3655-3662.2014_okoya__and_adekunle_et_al_.pdf
Olawepo", "KD, Banjo" "OT, Jimoh" "WA, Fawole" "WO, Orisasona" "O, Ojo-Daniel" "AH.  2014.  Effect of Cooking and Roasting on Nutritional and Anti- Nutritional Factors in Kenaf (Hibiscus Cannabinus L.) Seed Meal. Food Science and Quality Management. 24
MONDAY James, INNEH Godwin, OJOV.  2014.  Effect of Internal Control Systems on Operating Performance of Small Business in Lagos Metropolis. International Conference on Accounting, Finance and Management,. :237-256., Ile-Ife: Department of Management & Accounting, O.A.U.my_paper_internal_control_icafim_2014_full_paper_amended_1.pdf