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Objective:Pit and fissure sealants have been used for many decades to prevent the initiation of caries on susceptible tooth surfaces. The purpose of this study was to analyze the peer-reviewed published scientific literature on pit and fissure sealants over the last 50 years.
Materials and methods:
On the PubMed database, all publications on pit and fissure sealants from 1962-2011 were extracted using the search phrase [(pit OR fissure) AND (sealant OR sealants OR adhesive)]. Details of all retrievals were individually entered into SPSS for analysis.
Results:
A total of 2829 publications were found. The mean number of authors was 2.73 ± 1.90 (range = 1-23). Although single-authorship was the modal group with 32.1%, it had a sustained decrease from 75.0% for 1962-1971 to 17.6% for 2002-2011. On the contrary, publications with three or more authors increased from 8.3% to 47.3% during the same period. Human studies accounted for 88.6% and clinical trial was 11.9%, followed by reviews at 10.2% and randomized controlled trials at 6.9%. English was the language of reporting for 82.0% of the studies.
Conclusion:
It is anticipated that future research on pit and fissure sealants will focus on newer and more effective materials.

• Limited sunlight reduces plant productivity and foraging activities of pollinators, such as when alien trees shade out native flowering plants. The conservation management response is to remove the impoverishing effect of the alien tree canopy. However, alien trees can provide benefit for certain species when they provide significant scarce resources. We assess how flower-visiting insects respond to shading from young, small-sized, open canopy vs. mature, tall, closed-canopy alien pine trees retained specifically as refuges for certain rare mammals.• We sampled flower–visitor species diversity and interactions at various distances from pine stands of both sizes in a matrix of low sclerophyllous natural vegetation. We sampled flower–visitors using coloured pan traps and estimated flower–visitor interactions and flower diversity.
• Reduction in percentage light reaching the understorey significantly reduced flower abundance, with zero flowers in tall pine understorey. Flower–visitor species composition differed across sampling locations with increasing distance from tall pine understorey. Although pine tree size led to a decline in flower–visitor interaction frequency and network specialisation in all pine tree stands, some bee species that are mostly tree nesters, were observed in unique interactions in association with tall pines only.
• We show that alien pines, especially tall trees, have an impoverishing effect on flowering plants and flower–visitors. However, these trees also confer benefit to specialised groups such as tree-nesting bees. While active alien pine removal is encouraged where the trees are actively invading, maintaining well-contained small stands is of value for two different taxa of conservation concern.

We examined retrospectively 44 patients with refractory acute leukemia (acute myeloid leukemia (AML)/acute lymphoblastic leukemia=25/19) who underwent allogeneic transplantation at our center between 11/1990 and 04/2004. The median leukemic blasts was 25% and age 28 years (range, 3-56). Twenty-one patients had untreated relapse, 13 failed reinduction, eight in partial remission and two aplastic. Conditioning was myeloablative using cyclophosphamide, busulfan, total-body irradiation and etoposide (Bu/Cy/VP, n=22; TBI/Cy/VP, n=17; others, n=5) followed by marrow or peripheral blood transplant (n=23/21) from unrelated or related donors (n=28/16). All patients had graft-versus-host disease (GVHD) prophylaxis with cyclosporin and methotrexate. One patient experienced late graft failure. Severe acute-GVHD and chronic-GVHD appeared in eight and 14 patients, respectively. Thirteen patients (30%) remain alive after a median of 25.3 months (range, 2.4-134.1); with 31 deaths, mostly from relapse (n=15) and infections (n=12). Overall survival (OS) and progression-free survival (PFS) at 5 years was 28 and 26%, respectively. OS and PFS were significantly better with blasts < or =20% and time to transplant < or =1 year while transplant-related mortality was less with the use of TBI. We conclude that patients with refractory leukemia can benefit from allogeneic BMT, especially with < or =20% marrow blast.

AMPA receptors (AMPARs) are tetrameric ligand-gated channels made up of combinations of GluA1-4 subunits encoded by GRIA1-4 genes. GluA2 has an especially important role because, following post-transcriptional editing at the Q607 site, it renders heteromultimeric AMPARs Ca2+-impermeable, with a linear relationship between current and trans-membrane voltage. Here, we report heterozygous de novo GRIA2 mutations in 28 unrelated patients with intellectual disability (ID) and neurodevelopmental abnormalities including autism spectrum disorder (ASD), Rett syndrome-like features, and seizures or developmental epileptic encephalopathy (DEE). In functional expression studies, mutations lead to a decrease in agonist-evoked current mediated by mutant subunits compared to wild-type channels. When GluA2 subunits are co-expressed with GluA1, most GRIA2 mutations cause a decreased current amplitude and some also affect voltage rectification. Our results show that de-novo variants in GRIA2 can cause neurodevelopmental disorders, complementing evidence that other genetic causes of ID, ASD and DEE also disrupt glutamatergic synaptic transmission.